P3113-84E2
Pax7 (Paired Box Gene 7)
Size
£889.00
- SKU:
- P3113-84E2
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to aa1-196 from human Pax7 expressed in E. coli (P23759).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- PAX7 is a 57kD member of the paired homeobox family of transcription factors. It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin-cells) and a lineage commitment state (PAX7+ desmin+ cells). PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus. Human PAX7(A) is 520aa in length. It contains an N-terminal paired box DNA-binding domain (aa34-163), a homeodomain (aa218-275) and a C-terminal poly-alanine region (aa340-346). Multiple splice forms of PAX7 exist. There is a 54kD isoform B that contains a 38aa substitution for aa468-520, plus a second isoform that shows a deletion of Gly151 and Leu152. There is also a chromosomal rearrangement that fuses aa30-385 of PAX7 to aa211-265 of FOXO1A/FKHR. Over aa1-196, human PAX7 shares 86-96aa identity with human PAX3 and mouse PAX7. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice