PAX5 (Paired Box Protein Pax-5, B Cell-specific Transcription Factor, BSAP, PAX-5)

Size

£642.00

SKU:: P3113-83V1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to DLEKNYPTPRTSR-C, from human PAX5, at N-terminal (NP_057953.1). Species sequence homology: mouse
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Pax-5 is a 46kD, B cell specificactivator protein (BSAP). In the early stages of B cell developmemt, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20. Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. This indicates that Pax-5 is important in B cell development, but may also have a role in proper neuronal development. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64,000 Western Blot: 0.3-1ug/ml, human lymph and human spleen lysates observed on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice