Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1, Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1) (MaxLight 490)

Size

£927.00

SKU:: P3113-82C4-ML490
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1, conjugated to KLH.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Extra Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Patched 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml detected Patched 1 on 10ug of human skeletal muscle, fetal tissue lysate. Immunohistochemistry (Paraffin): 1:300 detected Patched 1 in kidney tissue. Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Positive Control: Human skeletal muscle, fetal tissue lysate. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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