Parvin, alpha

Size

£876.00

SKU:: P3113-60A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant human alpha-Parvin Val46-Phe133, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Alpha-Parvin (parvus [Latin for “small”]-A/ alpha; also actopaxin and PARV-A) is a 53kD member of the parvin family, alpha-actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372aa in length. It contains two CH/calponin homology domains (aa99-202 and 265-369), two NLSs (aa19-26 and 38-40), and a recently identified paxillin binding sequence (aa248-256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21aa substitution for aa239-372, and a second also showing a 46aa substitution for aa 134-372, respectively. Over aa46-133, human and mouse PARVA share 95% aa identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice