P3113-51

Parvalbumin (PARV)

Size

£927.00

SKU:: P3113-51
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified frog muscle Parvalbumin
Clone:: 3H43
Format:: Supplied as a liquid in PBS, 0.05% sodium azide
Extra Details:: Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Parvalbumin (PARV, PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PARV has two isoforms: alpha (rat/mouse/human: 110aa, 7p13-p11) and beta form (mouse/rat/human: 109aa) shares ~48% identity (mouse PARVs). The PARV-alpha is less acidic (pI=5) than the beta isoform and has an additional amino acid in the C-terminal helix. PARV-alpha is also expressed in inner ear. PARV-beta isoform is expressed in many fetal tissues, placenta and tumors. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Reactivity is Calcium ion dependent. Western Blot: 1:2000-5000 (ECL). MW: ~12kD. Immunohistochemistry & Immunofluorescence: 1:500-1:1000 in indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice