P3113-22
PARP 1, cleaved (Asp214) (Poly ADP-Ribose Polymerase 1, Poly [ADP-ribose] Polymerase 1, PARP1, PARP-1, PARP, ADPRT 1, ADPRT1, ADPRT, NAD(+) ADP-ribosyltransferase 1, pADPRT-1, Poly[ADP-ribose] Synthase 1, PPOL)
Size
£416.00
- SKU:
- P3113-22
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Asp214 in human PARP.
- Clone:
- 11C1057(D64E10)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Extra Details:
- PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24kD) from the carboxy-terminal catalytic domain (89kD) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200-1:800 Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice