P3113-06D1
PARP (Poly ADP-Ribose Polymerase, ADP Ribosyltransferase NAD+, ADPRT, ADPRT1, NAD(+) ADP Ribosyltransferase 1, pADPRT-1, Poly Adenosine Diphosphate ADP Ribose Polymerase, Poly [ADP-ribose] Polymerase 1, PARP1, PARP-1, Poly (ADP-ribose) Polymerase Family Member 1, Poly [ADP-ribose] Synthetase 1, PPOL) [KO Validiated]
Size
£320.00
- SKU:
- P3113-06D1
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant protein corresponding to N-terminal 2/3 sequence of PARP.
- Clone:
- 5A5
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- PARP (Poly (ADP-ribose) polymerase) is a 113kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml (1:500-1:1000) Immunocytochemistry: 1-5ug/ml Immunohistochemistry (paraffin): 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice