P3113-03A
PARP (214, 215) Cleavage Site
Size
£997.00
- SKU:
- P3113-03A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP. Species sequence homology: mouse, rat and hamster (86%)
- Format:
- Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
- Extra Details:
- Poly (ADP-Ribose) Polymerase (PARP) is a 116kD nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85kD and a 25kD fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat and HeLa cells treated with staurosporine or etoposide (25uM for 3 hours) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice