P3112-98D

Poly ADP-Ribose (PAR)

Size

£927.00

SKU:: P3112-98D
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified bovine Purified poly (ADP-ribose) polymer
Clone:: 7C43
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate. Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity. Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. TANK1 is alternatively spliced to isoform 1 and 2 (missing 644-1327). The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers. TANK1 has 24 ankyrin repeats in TRF-1 interacting domain near the N-terminus. The 33-aa ANK repeat motif mediates protein-protein interactions. The ANK domain is followed another protein interaction motif called the sterile alpha-module (SAM). The C-terminal region of TANK1 contains the PARP activity. TANK1 uses its ANK domain to bind TRF1 and its PARP domain to ADP-ribosylate itself and TRF1, and thereby inhibiting the ability of TRF1 to bind telomere. The homology between tankyrase and PARPs is limited to catalytic domain. Takyrase-1 is expressed in many tissues and targeted to various intracellular compartments. Tanyrase-1, devoid of NLS (nuclear localization signal), is translocated to telomere (nucleus) through binding of its ANK domain to TRF1. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL technique. See P3113-08A. ELISA: 0.5-1ug/ml; Control proteins can be used to coat ELISA plates at 1ug/ml and detected with antibodies. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice