P3112-03E

Parkin (RING-type E3 ubiquitin-protein ligase)

Size

£761.00

SKU:: P3112-03E
Application:: WB
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Val437 from human Parkin isoform 2, expressed in E. coli.
Clone:: 7H90
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Parkin is a 465 amino acid protein with an N-terminal ubiquitin-like domain, a linker region, a C-terminal TRIAD domain consisting of two RING finger motifs flanking a cysteine-rich IBR (in between RING fingers) motif. Mutations in Parkin are a major cause of autosomal recessive juvenile parkinsonism. Parkin-2 lacks exon 5, which encodes amino acid residues 179-206 of Parkin. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with recombinant human Parkin Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.