P3112-03D
Parkin (RING-type E3 ubiquitin-protein ligase)
Size
£735.00
- SKU:
- P3112-03D
- Purity:
- Tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length recombinant human Parkin
- Clone:
- 6A158
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- Parkin is a ubiquitin-protein isopeptide ligase (E3) involved in ubiquitin/proteasome-mediated protein degradation. Parkin may protect cells from premature death by targeting misfolded or damaged proteins for degradation via the ubiquitin proteasome pathway. Mutations in the parkin gene cause a loss-of-function and/or alter protein levels of parkin. The loss of functional parkin is the most common single cause of autosomal recessive Parkinson’s disease.1-2 Parkin expression is neuron-specific and developmentally regulated, and thus, has a role in neuronal maturation. Parkin may also play roles in cell cycle regulation and tumor suppression.3 Applications: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:1000 Immunoprecipitation: 100ul per test Western Blot: 1:20-1:100 Immunofluorescence: 1:20-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice