P3112-02M
Parkin (AR-JP, E3 Ubiquitin Protein Ligase Parkin, FRA6E, LPRS2, Parkinson Disease (Autosomal Recessive, Juvenile) 2, Parkinson Disease Protein 2, Parkinson Juvenile Disease Protein 2, PARK2, PRKN 2, PRKN, PDJ, RING-type E3 Ubiquitin-protein Ligase, Ubiquitin E3 Ligase PRKN) (Not for Export EU)
Size
£735.00
- SKU:
- P3112-02M
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to RILGEEQYNRYQQYGAEEC of human parkin conjugated to diphtheria toxoid.
- Format:
- Supplied as a lyophilized powder. Reconstitute with 100ul of sterile distilled water. Centrifuge to remove insoluble material. No preservative added.
- Extra Details:
- Parkinson’s disease, a common neurodegenerative disease is caused by slow death of neurons in the substantial nigra, a brain region that utilizes the neurotransmitter dopamine. Parkin, a recently discovered gene encoding a large protein, may be involved in normal and abnormal protein degradation in cells. Recent evidence indicates that point mutations in the Parkin gene appears to be responsible for the pathogenesis of some forms of Parkinson’s disease. The Parkin protein is expressed in various regions of the brain, including the substantia nigra, and is also expressed in other tissues, including heart, testes and skeletal muscle. This protein may function in the control of cell growth, differentiation and development. Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box. In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 (50kD band was identified in rat brain extract). Immunohistochemistry (frozen): 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice