Parathyroid hormone receptor 1 (PTH/PTHr receptor, PTH1 R)

Size

£856.00

SKU:: P3109-51C
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Tyr23-Met189 from human PTH1 R extracellular domain expressed in CHO cells. Species Sequence Homology: mouse, 88%
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: PTH1 R (Parathyroid hormone receptor 1; also PTH/PTHr receptor) is a predicted 66-68kD member of the G-protein coupled receptor family 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca ++ -release from bone, mediated by osteoclast formation. Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml HUVEC human umbilical vein endothelial cells Immunohistochemistry: 5-15ug/ml. Immersion fixed paraffin-embedded sections of human kidney sibjectde to Antigen Retreival Reagent-Basic Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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