P3107-80
Paraoxonase 3 (PON3, Lactonase 3)
Size
£825.00
- SKU:
- P3107-80
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Ala30-Leu354 of human PON3 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6kD protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn-323. Alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-28C: IgG, H&L (HRP) Pab Dk x Gt I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab Dk x Gt Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice