P3105-75E
PAR2 (Proteinase Activated Receptor 2)
Size
£889.00
- SKU:
- P3105-75E
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human PAR-2 expressed in NS0 cells.
- Clone:
- 7H40
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Proteinase-Activated Receptor 2 (PAR-2) is a proteinase activated 7-transmembrane G-protein-coupled receptor. Human PAR-2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361aa receptor. PAR-2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR-2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR-2 shows 78% amino acid identity with mouse PAR-2 over the extracellular portions. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells tested with HT-29 human colon adenocarcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice