PAR1 (Proteinase Activated Receptor 1)

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£863.00

SKU:: P3105-60K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant human PAR1 (aa27-102; 375-428)
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 45kD, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody was tested in flow cytometry using whole blood granulocytes. Dilute this antibody to 50ug/ml and add 10 μL of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 0.2 ng/well. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice