P3105-23B-FITC
Papilloma Virus, Human, Type 18, E6 (hPV, Human Papillomavirus Type 18 E6, E6, Early Protein E6, HpV18gp1, Protein E6) (FITC)
Size
£971.00
- SKU:
- P3105-23B-FITC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Gel-purified HPV 18 E6-beta galactosidase fusion protein.
- Clone:
- 9L397
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. HPV early proteins E6 and E7 are the major viral oncoproteins that regulate cell proliferation through the inactivation of p53 and Rb1 tumor suppressor proteins respectively. BF7 can be used for detection of HPV in cervical smears and biopsies and analysis of E6 expression in cell transformation studies. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin fixed, paraffin embedded sections. E6 levels too low for Immunofluorescence. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice