P3105-07F-ML550
Papilloma Virus, Human, Type 16, Early Protein 7 (hPV) (MaxLight 550)
Size
£1,004.00
- SKU:
- P3105-07F-ML550
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HPV 16 E7 synthesized in S. pombe.
- Clone:
- 9L394
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen section), Flow Cytometry, and RIA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice