P2488-15E
PAK1/2/3, phosphorylated (Thr423/Thr402/Thr421) (p21 Activated Kinase 1,2,3)
Size
£359.00
- SKU:
- P2488-15E
- Application:
- IHC-Fr
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to human PAK1 around the phosphorylation site of threonine 423 (R-S-TP-M-V).
- Format:
- Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The PAKs are a highly conserved family of serine-threonine protein kinases that bind to and are autophosphorylated and activated by small GTPases. Alpha-PAK (PAK-1) and beta-PAK (PAK-3) are mostly expressed in the brain while gamma-PAK (PAK-2) is expressed ubiquitously. In mammals, PAKs regulate the MAPK pathways and cytoskeletal organisation by increasing actin and tubulin polymerisation. PAKs also phosphorylate steroid receptors such as the estrogen and progesterone receptors. In humans the loss of function of PAK-3 is associated with X-linked, nonsyndromic mental retardation. Various phosphorylation sites exist on the PAKs, phosphorylation of Thr402 is essential for kinase activity. It should be noted that Thr402 in PAK-2 corresponds to Thr423 in PAK-1. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice