P2488-05

PAK 1 PBD (Rac/cdc42) Assay Reagent, Recombinant, Human (Agarose)

Size

£544.00

SKU:: P2488-05
Purity:: ≥90% (SDS-PAGE and Coomassie blue staining)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid suspension, bound to glutathione agarose beads; provided as a 50% slurry in PBS, pH 7.4, 50% glycerol.
Extra Details:: GST-tagged fusion protein to residues 67-150 of human PAK-1, expressed in E. coli, bound non-covalently to glutathione-agarose. Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates. Species Crossreactivity: Predicted to interact with all mammalian Rac and cdc42. Applications: Suitable for use in Affinity Precipitation and Immmunoblot. Other applications have not been tested. Recommended Dilutions: Affinity Precipitation: 10ug precipitated Rec-GTP and cdc42-GTP fromn HeLa cell lysates that were loaded in vitro with GTP-gammaS and GDP. Immunoblot: 1ug/ml Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe