P2488-01A

PAK1 (p21 GTPase activated protein kinase 1)

Size

£524.00

SKU:: P2488-01A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: E. coli-expressed human PAK1 (aa 333-545)
Format:: Supplied as a lyophilized powder, 0.09% sodium azide. Reconsitute with 200ul sterile PBS.
Extra Details:: Human p21-GTPase activated protein kinase 1 (PAK1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death against Bad. Membrane targeting of PAK1 induces neurite outgrowth. PAK1 may also regulate NF-kB activation. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.