Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2)

Size

£863.00

SKU:: P2150-70
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Mouse myeloma cell line NS0-derived recombinant mouse CD99, Gly25-Leu138
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: PILR-L (also known as CD99 and MIC2) is a 125 amino acid, 24-30 kD glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. PILR-L has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse PILR-L protein used as immunogen is 45% identical to the human counterpart. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 2 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 0.1 ng/well. In this format, this antibody shows less than 1% crossreactivity with rmPILR-α and rmPILR-β. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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