P1790-35
PACAP-Related Peptide (Pituitary Adenylate Cyclase Activating Polypeptide Related Peptide) (Not for Export EU)
Size
£1,088.00
- SKU:
- P1790-35
- Application:
- IF
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic rat PRP (Pensinsula), conjugated to BSA
- Format:
- Supplied as a lyophilized powder. Rconstitute with 100ul sterile ddH2O.
- Extra Details:
- Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. PACAP-related peptide (PRP) is a 29aa peptide, which is produced together with PACAP-27 and PACAP-38 when Prepro PACAP is being processed. PRP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining, while PACAP-27, PACAP-38, VIP, PHI, CRF, oxytocin and vasopressin do not. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen sections): 1:400-1:800 with overnight incubation at 2-8°C. Optimal dilutions to be determined by the researcher. Positive Control: Stefanini-fixed frozen sections of rat duodenum or hypothalamus. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice