PACAP (Pituitary Adenylate Cyclase Activating Polypeptide)

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£863.00

SKU:: P1774-95C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant corresponding to mouse PACAP expressed in E. coli (070176).
Format:: Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Extra Details:: PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17kD (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and a-MSH. Mouse PACAP proprecursor is 151aa in length (aa25-175). It undergoes proteolytic processing to generate two C-terminal amidated peptides termed PACAP-38 (aa131-168) and PACAP-27 (aa131-157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over (aa25-175), mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice