PA28, gamma, NT (Proteasome Activator 28-gamma subunit)

Size

£460.00

SKU:: P1710-05
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the N-terminal region of the human, mouse, rat and porcine PA28g (proteosome activator 28g, Ki antigen) protein. Species sequence homology: mouse, rat and porcine.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Proteasome activator 28 Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.(PA28), also known as 11S regulator, is composed of two homologous subunits, alpha (a) and beta (b), that share ~ 50% amino acid identity.1 A separate but related protein, PA28gamma, was originally identified as Ki antigen from sera of patients with systemic lupus erythematosus.1-3 PA28a and PA28b are located primarily in the cytoplasm, whereas PA28g is located in the nucleus.4,5 PA28 binds to the outer rings on both ends of the 20S proteasome to form a football-like structure.6,7 Binding of PA28 greatly stimulates multiple peptidase activities of the 20S proteasome in an ATP-independent reaction, but lacks the ability to degrade large protein substrates, suggesting that PA28 may cooperate with the 26 S proteasome in a sequential proteolytic pathway.8,9 Abnormally high expression of PA28g has been observed in thyroid cancer, particularly in regions of cancer cells where growth rate is accelerated.11 PA28g interacts directly with Hepatitis C virus (HCV) core protein, which becomes retained in the cell’s nucleus,12 and with MEKK3, which phosphorylates PA28g and increases its cellular levels.13 Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml. Immunoprecipitation: 7ug/reaction Optimal dilutions to be determined by the researcher. Positive Control: human MeWo malignant melanoma, A375 malignant melanoma, Jurkat T-lymphocyte. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice