P1005-10
p300
Size
£927.00
- SKU:
- P1005-10
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein containing the C-terminus of human p300
- Clone:
- 7C42
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- The transcriptional coactivator and integrator p300 and its closely related family member CREBBP mediate multiple signal-dependent transcriptional events. CREB binding protein (CBP) and p300 were both identified initially in protein interaction assays-the former through its association with the transcription factor CREB and the latter through its interaction with the adenoviral-transforming protein E1A. p300 contains 3 cysteine- and histidine-rich regions of which the most carboxy-terminal region interacts specifically with E1A. In its center, p300 contains a bromodomain, a hallmark of certain transcriptional coactivators. p300 and CREB-binding protein are highly related in primary structure. Several protein motifs such as a bromodomain, a KIX domain, and 3 regions rich in cys/his residues are well conserved between these 2 proteins. P300 protein is also a histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. Animals nullizygous for p300 died between days 9 and 11.5 of gestation, exhibiting defects in neurulation, cell proliferation, and heart development. Cells derived from p300-deficient embryos displayed specific transcriptional defects and proliferated poorly. These results provide evidence that a coactivator endowed with histone acetyltransferase activity is essential for mammalian cell proliferation and development. p300 is also a targeted by viral oncoproteins. it may also have a role in DNA repair synthesis through its interaction with proliferating cell nuclear antigen (PCNA). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 3-6ug/ml detected p300 in Hela nuclear cell extract (25-50ug protein). Immunoprecipitation: 5-10ug anti-p300 immunoprecipitated p300 from Hela nuclear extract. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice