P2X2 Receptor (Not for Export EU)

Size

£338.00

SKU:: P1003-05D
Purity:: Neat serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 13 amino acid peptide Synthetic peptide corresponding to amino acids 460-472 (DSTSDPKGLAQL) from the carboxy-terminus of the rat P2X2 Receptor protein.
Format:: Supplied as a liquid with 0.05% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot (Reducing): 1:1000 Cell membrane extracts were examined by SDS-Page, transferred to a nylon membrane. Membranes were blocked for 1 hour at 4˚C with 0.1% Tween 20 and 2.5% milk powder in PBS. Membranes were incubated with P1003-05D (1:500 in the same buffer overnight at 4˚C. Membranes were rinsed and incubated withI1904-47H: IgG, H&L (HRP) Pab GtxRt for 1 hour at RT. Following rinsing, the membranes were processed using ECL Immunohistochemistry: 1:1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. P1003-05D was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: 1:1000. P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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