P1001-97J

p130cas (Breast Cancer Anti-estrogen Resistance Protein 1, CRK-associated Substrate, Cas Scaffolding Protein Family Member 1, BCAR1, CAS, CASS1, CRKAS)

Size

£825.00

SKU:: P1001-97J
Application:: WB
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to aa66-412 from human p130Cas expressed in E.coli.
Clone:: 12K153
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: p130Cas is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93kD, it runs anomalously at 105-130kD in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31kD fragment promotes anoikis and cell death. Human p130Cas is 870aa in length. It contains one SH3 domain aa3-65, a Pro-rich region aa74-87, at least 10 phosphotyrosine aa128-410 and four phosphoserine aa34-639 sites, an SH3 binding motif aa635-643 and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22aa substitution for aa1-4, an alternate start site at Met30, a deletion of aa2-211, and a four aa substitution for aa1-4 with a deletion of aa65-212. Over aa66-412, human p130Cas shares 91% aa identity with mouse p130Cas. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice