p73b (Not for Export EU)

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£704.00

SKU:: P1001-40R
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: An 8 amino acid peptide sequence from the C-terminus of human p73b (KLH) (gene accession # BAB87245.1).
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: Human p73a is the first known structural and functional homolog of one of the most studied tumor protein, p53 (1). Human p73 gene encodes two distinct polypeptides: The p73 long form (human 636 AA, termed p73a; mouse 590 aa) and the short form (499 AA; termed p73b). The short form arises through alternative splicing of the p73. With the exception of C-terminal 5 AA, p73b is identical to p73a. Both p73a and p73b transcripts were detected all human tissues. The endogenous p73a protein has also been detected in cell extracts of HT-29, IMR-32, and SK-N-SH cells. Other cell lines, SK-N-MC SK-N-AS displayed significantly reduced levels of p73a protein (1). The C-terminal domain (364-393) of p53 and p73 are quite distinct. p73 has been mapped to human chromosome 1p36, a region that is frequently deleted in s variety of human cancers including neuroblastoma, colon cancer, melanoma, and breast cancer. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000. ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice