P1001-39H

p73

Size

£594.00

SKU:: P1001-39H
Purity:: Purified by Protein G affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73.
Clone:: 5B429
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide
Extra Details:: p73 was identified as a long-lost analog of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Applications: Suitable for use in ChIP, Western Blot, Immunoprecipitation, Immunofluorescence/Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ChIP: 1:10-1:500 Western Blot: 2ug/ml Immunoprecipitation: 1:10-1:500 Immunofluorescence (IC): 1:10-1:500 Immunohistochemistry (Frozen/Paraffin): 5-15ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice