P1001-39E

p73, delta-N

Size

£594.00

SKU:: P1001-39E
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Peptide corresponding to a portion of amino acid residues 1-50 of human dNp73. Species sequence homology: mouse (alanine at position 8 is replaced by methionine)
Clone:: 4H223 (38C674.2)
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: p73 was identified as a long-lost analog of tumor suppressor protein, p53 (1). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (2). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (3). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein (4). Recently, Yang et al. (2000) have cloned a p73 variant that lacks an amino-terminal transactivation domain, called DNp73 (5). The DNp73 message is transcribed from an alternative promoter located in intron 3. Applications: Suitable for use in ChIP, Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunocytochemistry (IF): 1:20-1:1000 Immunohistochemistry (frozen): 1:20-1:1000 Immunohistochemistry (paraffin): 5ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice