P1001-38X
P70 S6 Kinase beta (70kD Ribosomal Protein S6 Kinase 2, EC 2.7.11.1, p70 Ribosomal S6 Kinase beta, p70 S6Kbeta, p70-beta, p70-S6KB, Ribosomal Protein S6 Kinase beta-2, S6 Kinase-related Kinase, S6K-beta, S6K2, Serine/threonine-protein Kinase 14 beta, SRK)
Size
£832.00
- SKU:
- P1001-38X
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to aa2-410 from human P70 S6 Kinase expressed in E. coli (Q9UBS0).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- P70S6K b is a monomeric, 54kD member of the AGC family of Ser/Thr kinases. It is widely expressed, and found predominately in the nucleus. Although it was initially reported to phosphorylate the ribosomal S6 protein, p70S6Kb/S6Kb2 is now known to also phosphorylate RafB (cell survival), hnRNP (cell proliferation), p300/CBP (transcription) and CDC42 (cytoskeleton stability). Human p70S6Kb/S6Kb2 is 482aa in length. It contains a protein kinase domain (aa67-328), a C-terminal regulatory region (aa329-399) that contains an NLS (aa384-389), and a Pro-rich segment (aa411-482) that likely interacts with SH3 domain-containing proteins. Phosphorylation/dephosphorylation events and sumoylation regulate p70S6Kb activity. In addition to the 54kD form, there is a 56-60kD isoform that possesses a 13aa addition at the N-terminus, plus an isoform that shows a deletion of aa 267-270. Over aa1-410, human p70S6Kb2 shares 95-75aa identity with mouse p70S6Kb2 and human p70S6Kb1, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice