P1001-37F
p70 S6 Kinase (p70 S6K)
Size
£825.00
- SKU:
- P1001-37F
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues 26–43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- Applications: Suitable for use in Western Blot in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2.0ug/ml detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system Immunoprecipitation: 4ug immunoprecipitates p70 S6 kinase from 500ug of HEK293 RIPA lysate. Optimal dilution determined by the researcher. Immunoprecipitation Kinase Assay: 5ug immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice