P1001-37B1

p70 S6 Kinase, Recombinant, Human, GST-Tag (p70 S6K)

Size

£907.00

SKU:: P1001-37B1
Molecular Weight:: 74
Purity:: ≥60% by SDS-PAGE
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 3mM DTT, 30% glycerol.
Extra Details:: Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specific Activity: ~2pmol/min/ug Assay Conditions: Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35 and 2uM substrate (SER/THR 7) at RT for 1 hour. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.