P1001-32Z
p53 (Tumor Suppressor Protein, Oncogene Protein)
Size
£620.00
- SKU:
- P1001-32Z
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Gel-purified p53-beta-galactosidase fusion protein containing murine p53 aa14-389 from pSV53C cDNA clone. Epitope: aa213-217 on human p53. Cellular Localization: nuclear.
- Clone:
- 3C167 (PAb 240)
- Format:
- Supplied as a liquid in PBS, pH 7.2.
- Extra Details:
- p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas. Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene. The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression. p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000. Immunohistochemistry (Frozen): 1:250-1:500 Immunohistochemistry (Paraffin): 1:250-1:500; antigen retrieval is not essential but may optimize staining. Immunoprecipitation: 10ug/mg protein lysate. By IP this antibody reacts with only mutant p53 protein under non-denaturing condiions. Optimal dilutions to be determined by the researcher. Positive Control: MDA-MB-231 cell line Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice