P1001-29D2
p53 (Tumor Suppressor Protein, Oncogene Protein)
Size
£544.00
- SKU:
- P1001-29D2
- Purity:
- Culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human full-length wild type p53.
- Clone:
- 5G175
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:12.5 Use Ab 40ul/ml at RT for 2hours. Immunohistochemistry (Formalin/paraffin): 1:50. No pretreatment is necessary, however staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: MDA-231 cells, Colon carcinomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice