P1001-28F

p53 (Tumor Suppressor Protein, Oncogene Protein)

Size

£819.00

SKU:: P1001-28F
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human p53 (transcription domain within the NH2 terminus)
Clone:: Bp53-11
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for Immunohistochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen and paraffin-embedded tissue, cytological material): 1:500-1:1500 with PBS, pH 7.4, when using the APAAP method; Pretreatment: No pretreatment required. Do not apply proteases. Incubation Time 1 hr at 37°C, extended with paraffin sections (overnight at 2-8°C). APAAP, PAP or streptavidin/ biotin method or application of signal amplification system is strongly recommended. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.