P1001-26R-ML650
p53 (Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Cellular Tumor Antigen p53, Phosphoprotein p53, Antigen NY-CO-13, LFS1) (MaxLight 650)
Size
£852.00
- SKU:
- P1001-26R-ML650
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human p53.
- Clone:
- DO-1
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. p53 is a 53kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73. Applications: Suitable for use in Immunohistochemistry, FLISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen. Immunohistochemistry (Paraffin): 1:1000 Western Blot: 1:1000 Note: Requires antigen retrieval; sodium citrate buffer pH 6.0 is recommended . Recommended positive control tissue: Colon or breast carcanoma. Optimal dilutions to be determined by the researcher. Recommended Secondary Reagents: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Recommended Negative Controls (IHC): I1904-83P1: IgG2a Negative Control Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice