P1001-26G

p53, aa20-25 (Tumor Suppressor Protein, Oncogene Protein)

Size

£570.00

SKU:: P1001-26G
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human p53.
Clone:: DO-7
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required Immunohistochemistry (paraffin): 1:1000 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Immunohistochemistry: Frozen sections Western Blot: 1:1000 Optimal working dilutions to be determined by researcher. Positive Contol: Colon or breast carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.