P1001-26E

p53, mixed clones (Tumor Suppressor Protein, Oncogene Protein)

Size

£863.00

SKU:: P1001-26E
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human p53. MW Of Antigen: 53kD. Epitope: aa37-45 and aa20-25. Cellular Localization: Nuclear
Clone:: DO-7+BP53–12
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See P1001-26EX
Extra Details:: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunoprecipitation (Native and denatured): (Use Protein A; 2ug/mg protein lysate) Western Blot: 0.5-1ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minute at RT. Staining of formalin/paraffin tissues is ENHANCED by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min Optimal dilution determined by the researcher. Positive Control: MDA-231, SKBR-3 cells or Colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice