P1001-26D2
p53 (Tumor Suppressor Protein, Oncogene Protein)
Size
£512.00
- SKU:
- P1001-26D2
- Purity:
- Purified from ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human p53.
- Clone:
- 3G227 (PAB1801)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
- Extra Details:
- p53 is a 339 amino acid protein (53kD) that is thought to act as a tumor suppressor gene. p53 is a DNA-binding protein, and missense mutation of the gene can lead to loss of its DNA-binding functions, and subsequent inactivation of p53 may be a contributing factor in tumorigenesis. Mutation of p53 occurs at many sites and represents a common genetic alteration in human malignancy. Mutations which result in overexpression of p53 can be detected by immunohistochemistry, and overexpression and accumulation of p53 in cell nucleus was reported for a number of human tumors, such as breast, lung and colon carcinomas. Staining of p53 overexpression may be a useful tumor and prognostic marker. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: ~1:1000 Immunoprecipitation (Native): ~2–5ug Immunohistochemistry (Frozen or PE): ~1:50–100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice