p47 phox (NCF1, NOXO2, p47phox, Neutrophil Cytosolic Factor 1, 47kD, Chronic Granulomatous Disease, Autosomal 1, Neutrophil Cytosolic Factor-1 47kD)

Size

£642.00

SKU:: P1001-24J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide CSESTKRKLASAV, corresponding to C-terminal of human p47 phox. Species Sequence Homology: rat, rabbit, bovine
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1.5ug/ml, ~48kD band observed in lysates of cell line U937 and of mouse Thymus, and 50kD observed in porcine Spleen lysates Peptide ELISA: 1:20,000 Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See P1001-24J-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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