P1001-23B-BIOTIN

p45 (SKP2) (Biotin)

Size

£1,106.00

SKU:: P1001-23B-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full-length recombinant protein corresponding to human p45 (SKP2).
Clone:: 3G225
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Extra Details:: The F-box protein p45SKP2 is the substrate-targeting subunit of the protein ligase SCFSKP2 and is frequently overexpressed in transformed cells. Expression of p45SKP2 in quiescent fibroblasts promotes p27Kip1 degradation, allows the generation of cyclin-A-dependent kinase activity, and allows entry into the S phase of the cell cycle. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Positive Control: HeLa, Jurkat and 293T cell lines. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.