P1001-10A-ML650
p27kip1, phosphorylated (Thr187) (MaxLight 650)
Size
£971.00
- SKU:
- P1001-10A-ML650
- Purity:
- Purified by Protein A chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide encompassing the phosphorylated Thr187 residue of human p27. Species sequence homology: mouse, rat and hamster.
- Clone:
- 2B10B7
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The cyclin-dependent kinase inhibitor p27KIP1 is a potent inhibitor of both Cyclin E-Cdk2 and Cyclin A-Cdk2, two cdk complexes involved in the regulation of G1/S transition. p27 is phosphorylated on threonine 187 by cyclin E/Cdk2, and overexpression of this complex in mammalian cells induces degradation of transfected p27. The Cdk-mediated phosphorylation of p27 on threonine 187 is required for p27 ubiquitination and consequent degradation. The phosphorylation on threonine 187 and the ubiquitination of cellular p27, as well as p27 ubiquitinating activity, were found to be regulated collectively during the cell cycle, being present in proliferating cells but not in G0- and G1- arrested cells. Applications: Suitable for use in FLISA and Western Blot. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice