P1000-40H
p19skp1 (S-phase Kinase-associated Protein)
Size
£709.00
- SKU:
- P1000-40H
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human SKP1 (p19) protein.
- Clone:
- 3G222
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- The SKP1 gene was first identified in yeast, where it is essential for the G1/S and G2/M cell cycle transitions.1 Its protein product, Skp1 (p19), interacts with cyclin F directly and cyclin A indirectly, and is thought to link these key regulatory proteins of the cell cycle to ubiquitin-mediated degradation by the proteolytic pathway. Skp1 is a subunit of a type of E3 ubiquitin ligase known as an SCF ligase, for its Skp1, Cul1, and F-box protein components. Within the SCF complex, Cul1 functions as the catalytic core which recruits the E2 ubiquitin-conjugating enzyme, the F-box protein provides substrate specificity for the protein to be degraded,2 and Skp1 acts as an adapter protein linking the F-box protein to Cul1.3 De-regulation of SCF-dependent protein degradation has been implicated as a contributing factor in malignant transformation. The complete loss or increased proteolysis of cyclin E, b-catenin, and p27, all of which are degradation targets of specific SCF ligases, has been observed as a frequent event in cancer.3 The human SCF ligase containing Skp2 as its F-box protein (SCFSkp2) degrades p27, SCFTrcp targets b-catenin,4 and SCFFbw7/hCdc4/hAgo degrades cyclin E. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA (Immunogen): 0.1-1ug/mL Western Blot: 1-3ug/ml Immunoprecipitation: 5-10ug/ml Optimal dilutions to be determined by the researcher. positive controls: HeLa cervical carcinoma, Jurkat leukemia T cell and Wi-38 lung fibroblast cell lysates. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice