P1000-40H-BIOTIN
p19skp1 (S-phase Kinase-associated Protein) (Biotin)
Size
£965.00
- SKU:
- P1000-40H-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human SKP1 (p19) protein.
- Clone:
- 3G222
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- The SKP1 gene was first identified in yeast, where it is essential for the G1/S and G2/M cell cycle transitions.1 Its protein product, Skp1 (p19), interacts with cyclin F directly and cyclin A indirectly, and is thought to link these key regulatory proteins of the cell cycle to ubiquitin-mediated degradation by the proteolytic pathway. Skp1 is a subunit of a type of E3 ubiquitin ligase known as an SCF ligase, for its Skp1, Cul1, and F-box protein components. Within the SCF complex, Cul1 functions as the catalytic core which recruits the E2 ubiquitin-conjugating enzyme, the F-box protein provides substrate specificity for the protein to be degraded,2 and Skp1 acts as an adapter protein linking the F-box protein to Cul1.3 De-regulation of SCF-dependent protein degradation has been implicated as a contributing factor in malignant transformation. The complete loss or increased proteolysis of cyclin E, b-catenin, and p27, all of which are degradation targets of specific SCF ligases, has been observed as a frequent event in cancer.3 The human SCF ligase containing Skp2 as its F-box protein (SCFSkp2) degrades p27, SCFTrcp targets b-catenin,4 and SCFFbw7/hCdc4/hAgo degrades cyclin E. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA (Immunogen): 0.1-1.0ug/mL Western Blot: 1-3ug/ml Immunoprecipitation: 5-10ug/ml Optimal dilutions to be determined by the researcher. positive controls: HeLa cervical carcinoma, Jurkat leukemia T cell and Wi-38 lung fibroblast cell lysates. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice