P0900-01
p13, Myeloid Marker
Size
£927.00
- SKU:
- P0900-01
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes.
- Clone:
- BM3
- Format:
- Supplied as a liquid in pBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
- Extra Details:
- p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and nonlymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13,000 Dalton protein. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen sections) . The antibody does not stain tissue after paraffin treatment. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:100. Optimal dilution determined by the researcher. Hybridoma: BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice