O8490
Otubain 1, Recombinant Human, aa1-271, His-Tag (OTUB1, OTB1)
Size
£512.00
- SKU:
- O8490
- Molecular Weight:
- 33.4
- Purity:
- ≥95% by SDS-PAGE Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as liquid in 20mM Tris, pH 8.0, 10% glycerol.
- Extra Details:
- Otubain 1, also known as OTUB1, is a member of the OUT (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with a ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth. Recombinant OTUB1 protein , expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant human Otubain 1, aa1-271 His-tagged expressed in E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GTTNPHIFP EGSEPKVYLL YRPGHYDILY K Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice