O8490-02A

OTUB2 (Ubiquitin Thioesterase OTUB2, Deubiquitinating Enzyme OTUB2, OTU Domain-containing Ubiquitin Aldehyde-binding Protein 2, Otubain-2, Ubiquitin-specific-processing Protease OTUB2, C14orf137, OTB2, OTU2)

Size

£642.00

SKU:: O8490-02A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-YKTSHYNILYAADKH, from human OTUB2, at the internal region of the protein (NP_075601.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml, observed in human brain (cerebellum) lysates on ~27kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.