O8064-09C

Osteopontin (OPN, BNSP, Bone Sialoprotein 1, Bone Sialoprotein I, BSPI, Early T Lymphocyte Activation 1, ETA-1, HGNC:11255, MGC110940, Nephropontin, Secreted Phosphoprotein 1, SPP1, SPP-1, Urinary Stone Protein, Uropontin) (Carboxyfluorescein)

Size

£698.00

SKU:: O8064-09C
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C/-20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Osteopontin expressed in NSO cells.
Clone:: 11n145
Format:: Supplied as a liquid in 1ml saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein (CFS).
Extra Details:: Osteopontin (OPN), secreted phosphoprotein 1 (Spp1), and bone sialoprotein 1, is a secreted acidic phosphorylated glycoprotein originally isolated from bone matrix. It has been shown to bind to different cell types through interactions with various integrins and CD44 variants. It has important functions in bone metabolism and inflammatory processes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul per 5x10e5 cells. Optimal dilutions to be determined by the researcher. Absorption: 488nm Emission: 515-545nm Storage and Stability: Store in the dark at 4°C.. Aliquots are stable for 12 months after receipt at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Caution: CFS conjugates are sensitive to light.